Cross-peptide bond 13C15N coupling constants by 13C and J cross-polarization 15N NMR
نویسندگان
چکیده
منابع مشابه
Mode-Coupling Analysis of 15N CSA-15N-1H Dipolar Cross-Correlation in Proteins. Rhombic Potentials at the N-H Bond
15N CSA-15N-1H dipolar cross-correlation (ηxy) in proteins has been treated thus far with the model-free (MF) approach, where the global (RC) and local (RL) motions are assumed to be decoupled as a consequence of RL . RC. In the context of ηxy, it is additionally assumed that the local motion is very fast and highly symmetrical. We have recently applied to auto-correlated 15N spin relaxation th...
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The possibility of generating protein folds at the stage of backbone assignment using structural restraints derived from experimentally measured cross-hydrogen bond scalar couplings and secondary chemical shift information is investigated using as a test case the small alpha/beta protein chymotrypsin inhibitor 2. Dihedral angle restraints for the phi and psi angles of 32 out of 64 residues coul...
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UB, 13C, 14N, 15N NM R is used to study methylaminodiphenylborane (1). Compound 1 can be regarded as a model compound for studying BN(pp)jr-, B C (pp)7r interactions, for determining the barrier to rotation about the B —N bond and for the application of natural abundance 15N NM R to boron-nitrogen chemistry. The 13C NM R of 1 shows a large splitting of the uC (para) resonances (in contrast to r...
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Solid-state NMR technique for measuring distances between heteronuclei in static powder samples is described. It is based on a two-dimensional single-echo scheme enhanced with adiabatic cross polarization. As an example, the results for intramolecular distances in alpha-crystalline form of glycine are presented. The measured NMR distances (13)C(alpha)-(15)N and (13)C(')-(15)N are 1.496+/-0.002 ...
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ژورنال
عنوان ژورنال: Journal of Biochemical and Biophysical Methods
سال: 1979
ISSN: 0165-022X
DOI: 10.1016/0165-022x(79)90032-0